University of Wyoming researchers’ study of how microscopic creatures called tardigrades survive extreme conditions has led to a major breakthrough that could eventually make life-saving treatments available to people where refrigeration isn’t possible.
Thomas Boothby, an assistant professor of molecular biology, and colleagues have shown that natural and engineered versions of tardigrade proteins can be used to stabilize an important pharmaceutical used to treat people with hemophilia and other conditions without the need for refrigeration—even amid high temperatures and other difficult conditions. The findings are detailed in Scientific Reports.
The pharmaceutical known as human blood clotting Factor VIII is an essential therapeutic used to treat genetic disease and instances of extreme bleeding. Despite being critical and effective in treating patients in these circumstances, Factor VIII has a serious shortcoming, in that it is inherently unstable. Without stabilization within a precise temperature range, Factor VIII will break down.
“In underdeveloped regions, during natural disasters, during space flight or on the battlefield, access to refrigerators and freezers, as well as ample electricity to run this infrastructure, can be in short supply. This often means that people who need access to Factor VIII do not get it,” Boothby says. “Our work provides a proof of principle that we can stabilize Factor VIII, and likely many other pharmaceuticals, in a stable, dry state at room or even elevated temperatures using proteins from tardigrades—and, thus, provide critical lifesaving medicine to everyone everywhere.”
The human blood clotting cascade. The clotting cascade of human blood plasma follows two prominent pathways; intrinsic, measured by Activated Partial Thromboplastin Time (aPTT) and extrinsic, measured by Prothrombin Time (PT). To activate the intrinsic pathway, Human Blood Clotting Factor XII (FXII) acts as the first protein in a cascade of clotting factor activation. FXII activates FXI which activates FIX which finally activates FVIII. FVIII subsequently binds to and activates FX. To activate the extrinsic pathway, FVII forms a complex with Tissue Factor, activating FX. After activation of FX, both coagulation pathways converge. FX forms a complex with FV, converting prothrombin into thrombin. Thrombin then converts fibrinogen into fibrin, in turn creating a fibrin clot. Human plasma deficient in Factor VIII (highlighted in red) is unable to clot properly through the intrinsic pathway, unless supplemented with this factor, and thus clots more slowly. Adapted from Zaragoza and Espinoza-Villafuerte, 2017. © Scientific Reports (2023). DOI: 10.1038/s41598-023-31586-9
Measuring less than half a millimeter long, tardigrades—also known as water bears—can survive being completely dried out; being frozen to just above absolute zero (about minus 458 degrees Fahrenheit, when all molecular motion stops); heated to more than 300 degrees Fahrenheit; irradiated several thousand times beyond what a human could withstand; and even survive the vacuum of outer space. They are able to do so, in part, by manufacturing a sugar called trehalose and a protein called CAHS D.
According to the research paper, Boothby and his colleagues fine-tuned the biophysical properties of both trehalose and CAHS D to stabilize Factor VIII, noting that CAHS D is most suitable for the treatment. The stabilization allows Factor VIII to be available in austere conditions without refrigeration, including repeated dehydration/rehydration, extreme heat and long-term dry storage.
The researchers believe the same thing can be done with other biologics—pharmaceuticals containing or derived from living organisms—such as vaccines, antibodies, stem cells, blood and blood products.
“This study shows that dry preservation methods can be effective in protecting biologics, offering a convenient, logistically simple and economically viable means of stabilizing life-saving medicines,” Boothby says. “This will be beneficial not only for global health initiatives in remote or developing parts of the world, but also for fostering a safe and productive space economy, which will be reliant on new technologies that break our dependence on refrigeration for the storage of medicine, food and other biomolecules.”
Boothby and other researchers hope that their discoveries can be applied to address other societal and global health issues as well, including water scarcity. For example, their work might lead to better ways of generating engineered crops that can cope with harsh environments.
More information:
Maxwell H. Packebush et al, Natural and engineered mediators of desiccation tolerance stabilize Human Blood Clotting Factor VIII in a dry state, Scientific Reports (2023). DOI: 10.1038/s41598-023-31586-9. www.nature.com/articles/s41598-023-31586-9
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Citation:
Scientists use tardigrade proteins for human health breakthrough (2023, March 20)