Have you been hearing about the dire wolf lately? Maybe you saw a massive white wolf on the cover of Time magazine or a photo of “Game of Thrones” author George R.R. Martin holding a puppy named after a character from his books.
The dire wolf, a large, wolflike species that went extinct about 12,000 years ago, has been in the news after biotech company Colossal claimed to have resurrected it using cloning and gene-editing technologies. Colossal calls itself a “de-extinction” company. The very concept of de-extinction is a lightning rod for criticism. There are broad accusations of playing God or messing with nature, as well as more focused objections that contemporary de-extinction tools create poor imitations rather than truly resurrected species.
While the biological and philosophical debates are interesting, the legal ramifications for endangered species conservation are of paramount importance. As a legal scholar with a Ph.D. in wildlife genetics, my work focuses on how we legally define the term “endangered species.” The use of biotechnology for conservation, whether for de-extinction or genetic augmentation of existing species, promises solutions to otherwise intractable problems. But it needs to work in harmony with both the letter and purpose of the laws governing biodiversity conservation.
All that’s left of dire wolves today are bones, like these skulls on display in a museum.
Patrick T. Fallon/AFP via Getty Images
Of dire wolves and de-extinction
What did Colossal actually do? Scientists extracted and sequenced DNA from Ice Age-era bones to understand the genetic makeup of the dire wolf. They were able to piece together around 90% of a complete dire wolf genome. While the gray wolf and the dire wolf are separated by a few million years of evolution, they share over 99.5% of their genomes.
The scientists scanned the recovered dire wolf sequences for specific genes that they believed were responsible for the physical and ecological differences between dire wolves and other species of canids, including genes related to body size and coat color. CRISPR gene-editing technology allows scientists to make specific changes in the DNA of an organism. The Colossal team used CRISPR to make 20 changes in 14 different genes in a modern gray wolf cell before implanting the embryo into a surrogate mother.
While the technology on display is marvelous, what should we call the resulting animals? Some commentators argue that the animals are just modified gray wolves. They point out that it would take far more than 20 edits to bridge the gap left by millions of years of evolution. For instance, that 0.5% of the genome that doesn’t match in the two species represents over 12 million base pair differences.
More philosophically, perhaps, other skeptics argue that a species is more than a collection of genes devoid of environmental, ecological or evolutionary context.
Colossal, on the…
