Sewage-testing robots process wastewater faster to predict COVID-19 outbreaks sooner
The Research Brief is a short take about interesting academic work.
The big idea
By using a sewage-handling robot, our laboratory has been able to detect coronavirus in wastewater 30 times faster than nonautomated large-scale systems. This advance, published in the microbiology journal mSystems, provides even more lead time to communities monitoring their wastewater for early warning about local cases of COVID-19.
When clinical studies emerged showing that people who test positive for SARS-CoV-2 shed the virus in their stool, the sewer seemed like an obvious place to look for it. Wastewater surveillance can be used at the community level to see potential outbreak clusters before clinical diagnosis, especially in areas where COVID-19 prevalence rates far exceed testing rates.
The problem is that the virus is heavily diluted in the waste stream because of how many people’s bathrooms drain into it, not to mention all the other junk they flush. Surveillance depends on concentrating the viral particles from the wastewater to detect these low levels. This viral concentration step is typically the major bottleneck in wastewater analyses because it’s laborious and time-consuming. Our robot system takes a different, quicker approach.
Why it matters
Cities, schools and businesses around the country are using wastewater surveillance to find coronavirus in their midst.
Wastewater surveillance is especially useful as an early-alert system for high-risk areas, such as communities where undocumented residents may be cautious about individual testing.
The most commonly used viral concentration technique uses filters and can take anywhere from six to eight hours to transform a couple dozen sewage specimens into samples that can then be tested for the presence of SARS-CoV-2. Our new protocol concentrates 24 samples in a single 40-minute run.
We repurposed gear that usually performs microbiology or cell biology tasks in the lab to deal with sewage instead. By miniaturizing and automating our system, we eliminate a bunch of labor-intensive steps, resources and associated costs. And our hands-free process is much quicker.
How we do this work
We gather sewage from autosamplers at San Diego’s main wastewater treatment plant, as well as from those we’ve deployed at over 100 manholes on the campus of the University of California, San Diego, which collect sewer samples every 30 minutes through the day.
Using a robot to automate the sewage concentration process lets us concentrate 24 samples in 40 minutes for each robot. Then the same robot can extract the viral RNA, processing 96 samples in 36 minutes. Finally, we use a polymerase chain reaction to search for the signature genes of SARS-CoV-2, much like a clinical diagnostic test that a lab would run on a patient’s nasal swab.